Microbial Decolourization of Crystal Violet by Bacillus subtilis
نویسندگان
چکیده
The physicochemical characterization of the textile industry effluent collected from Oswal Textile Industries, Ludhiana (Punjab.) India has been carried out and the results showed that the temperature (40°C), pH (8.00), Biological Oxygen Demand (260 mgl –1 ), Chemical Oxygen Demand (790 mgl –1 ), Total Suspended Solids (2000 mgl –1 ), Total Dissolved Solids (7000 mgl –1 and colour over the prescribed fresh water limits. A potential bacterial strain was isolated and selected from the textile effluent on the basis of rapid azo dye Crystal violet (100mgl –1 ) decolorization and later identified as belonging to genus Bacillus based on Phenotypic characterization and phylogenetic analysis of the 16s rRNA gene sequence. Effects of physicochemical parameters (pH, Temperature, etc.) on the Crystal violet decolorization by the Bacillus were studied. Decolorization was effective at pH 8, 35°C with starch and peptone as carbon and nitrogen sources and in static conditions. This decolorization potential increased the applicability of this microorganism for the dye removal.
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